224 Skin layers targeting by multi-lamellar liposomes; interest for drug delivery in atopic dermatitis

In the frame of atopic dermatitis (AD), epidermis restricted delivery corticosteroids represents a promising strategy for drug efficiency improvement, blood transfer diminution and therefore side effects decrease. Multi-lamellar liposomes (MLLs) controlled size, charge elasticity are good candidates to reach such purposes. Their entrapment hydrophilic hydrophobic molecules ability plain MLLs remain in chosen skin layers has been demonstrated on Strat-M™ (an artificial model) dog skin. this study, we that after corticosteroid encapsulation still able control physico-chemical properties target epidermal layer model. Using Franz cell, 33 % free crossed 48 h against less than 2 encapsulated form. explants, showed corticosteroid-loaded did not alter architecture, confirmed decreased transdermal passage corticosteroids. Existing models reconstructed made polycarbonate filter thus lack dermis. To evaluate targeting MLLs, developed new model dead deepidermized After 48h exposure cytokines (Il-4, IL-13, IL-17), our an increased thickness, decrease filaggrin, loricrin staining, increase carbonic anhydrase staining which main characteristics AD. Thus, is suitable test reverse cytokines-induced alteration.